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fluoroshield tm with dapi  (Beyotime)


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    Structured Review

    Beyotime fluoroshield tm with dapi
    Fluoroshield Tm With Dapi, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 32539 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluoroshield+tm+dapi/DAPI/pmc11237085-260-63-67
    Average 99 stars, based on 32539 article reviews
    fluoroshield tm with dapi - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Blocking Assay:

    Article Title: Histamine H 1 receptors in dentate gyrus-projecting cholinergic neurons of the medial septum suppress contextual fear retrieval in mice
    Article Snippet: .. After blocking by 5% normal donkey serum in PBS for 1 h at room temperature, sections were incubated with anti-ChAT antibody (Millipore, AB144P, 1:100), anti-c-Fos antibody (Synaptic System, 226004, 1:500), at 4 °C overnight and then with AlexaFluor-conjugated secondary antibody-Donkey anti-Goat Alexa Fluor 488 (Invitrogen, A32814, 1:400) or Goat Anti-Guinea pig Alexa Fluor 647 (Abcam, ab150187, 1:400) at room temperature for 2 h. Fluoroshield TM with DAPI (Beyotime, P0131) was used as a nuclear stain. .. The slices were photographed by Olympus FV3000 confocal microscope and further quantified by FIJI (ImageJ-win64) in a blind manner.

    Incubation:

    Article Title: Histamine H 1 receptors in dentate gyrus-projecting cholinergic neurons of the medial septum suppress contextual fear retrieval in mice
    Article Snippet: .. After blocking by 5% normal donkey serum in PBS for 1 h at room temperature, sections were incubated with anti-ChAT antibody (Millipore, AB144P, 1:100), anti-c-Fos antibody (Synaptic System, 226004, 1:500), at 4 °C overnight and then with AlexaFluor-conjugated secondary antibody-Donkey anti-Goat Alexa Fluor 488 (Invitrogen, A32814, 1:400) or Goat Anti-Guinea pig Alexa Fluor 647 (Abcam, ab150187, 1:400) at room temperature for 2 h. Fluoroshield TM with DAPI (Beyotime, P0131) was used as a nuclear stain. .. The slices were photographed by Olympus FV3000 confocal microscope and further quantified by FIJI (ImageJ-win64) in a blind manner.

    Staining:

    Article Title: Histamine H 1 receptors in dentate gyrus-projecting cholinergic neurons of the medial septum suppress contextual fear retrieval in mice
    Article Snippet: .. After blocking by 5% normal donkey serum in PBS for 1 h at room temperature, sections were incubated with anti-ChAT antibody (Millipore, AB144P, 1:100), anti-c-Fos antibody (Synaptic System, 226004, 1:500), at 4 °C overnight and then with AlexaFluor-conjugated secondary antibody-Donkey anti-Goat Alexa Fluor 488 (Invitrogen, A32814, 1:400) or Goat Anti-Guinea pig Alexa Fluor 647 (Abcam, ab150187, 1:400) at room temperature for 2 h. Fluoroshield TM with DAPI (Beyotime, P0131) was used as a nuclear stain. .. The slices were photographed by Olympus FV3000 confocal microscope and further quantified by FIJI (ImageJ-win64) in a blind manner.



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    Image Search Results


    ZO-1 immunolabelling in the ileal mucosa. ( a – e ) Representative images of ZO-1 expression (green). Nuclei are counterstained with DAPI (blue). ( f ) Densitometric analysis of ZO-1-positive area. Exposure to hyperoxia significantly decreased ZO-1 expression compared to the normoxic controls ( a , c , f ). The treatment with BRL37344 at 3 mg/kg, but not at 1 mg/kg, had a protective effect ( c – f ). No significant changes were observed in the normoxia-exposed pups, treated or not with BRL37344 ( a , b , f ). Values are expressed as mean ± S.D. *** p < 0.001 hyperoxia versus normoxia; °°° p < 0.001 hyperoxia + BRL37344 versus normoxia; §§§ p < 0.001 hyperoxia + BRL37344 versus hyperoxia. Scale bar = 50 µm.

    Journal: Antioxidants

    Article Title: Protective Effects of Beta-3 Adrenoceptor Agonism on Mucosal Integrity in Hyperoxia-Induced Ileal Alterations

    doi: 10.3390/antiox13070863

    Figure Lengend Snippet: ZO-1 immunolabelling in the ileal mucosa. ( a – e ) Representative images of ZO-1 expression (green). Nuclei are counterstained with DAPI (blue). ( f ) Densitometric analysis of ZO-1-positive area. Exposure to hyperoxia significantly decreased ZO-1 expression compared to the normoxic controls ( a , c , f ). The treatment with BRL37344 at 3 mg/kg, but not at 1 mg/kg, had a protective effect ( c – f ). No significant changes were observed in the normoxia-exposed pups, treated or not with BRL37344 ( a , b , f ). Values are expressed as mean ± S.D. *** p < 0.001 hyperoxia versus normoxia; °°° p < 0.001 hyperoxia + BRL37344 versus normoxia; §§§ p < 0.001 hyperoxia + BRL37344 versus hyperoxia. Scale bar = 50 µm.

    Article Snippet: The specimens were washed with PBS and mounted in DAPI anti-fade aqueous medium (Fluoroshield TM with DAPI, Thermo Fisher Scientific).

    Techniques: Expressing

    E-cadherin immunolabelling in the ileal mucosa. ( a – e ) Representative images of E-cadherin expression (green; nuclei are counterstained blue with DAPI). ( f ) Densitometric analysis of E-cadherin-positive area. Exposure to hyperoxia significantly decreased E-cadherin expression compared to the normoxic controls ( a , c , f ). The administration of BRL37344 had a partially protective effect ( c – f ). No changes can be observed in the normoxic pups treated or not with BRL37344 ( a , b , f ). Values are expressed as mean ± S.D. *** p < 0.001 hyperoxia versus normoxia; °° p < 0.01 and °°° p < 0.001 hyperoxia + BRL37344 versus normoxia; §§ p < 0.01 hyperoxia + BRL37344 versus hyperoxia. Scale bar = 25 µm.

    Journal: Antioxidants

    Article Title: Protective Effects of Beta-3 Adrenoceptor Agonism on Mucosal Integrity in Hyperoxia-Induced Ileal Alterations

    doi: 10.3390/antiox13070863

    Figure Lengend Snippet: E-cadherin immunolabelling in the ileal mucosa. ( a – e ) Representative images of E-cadherin expression (green; nuclei are counterstained blue with DAPI). ( f ) Densitometric analysis of E-cadherin-positive area. Exposure to hyperoxia significantly decreased E-cadherin expression compared to the normoxic controls ( a , c , f ). The administration of BRL37344 had a partially protective effect ( c – f ). No changes can be observed in the normoxic pups treated or not with BRL37344 ( a , b , f ). Values are expressed as mean ± S.D. *** p < 0.001 hyperoxia versus normoxia; °° p < 0.01 and °°° p < 0.001 hyperoxia + BRL37344 versus normoxia; §§ p < 0.01 hyperoxia + BRL37344 versus hyperoxia. Scale bar = 25 µm.

    Article Snippet: The specimens were washed with PBS and mounted in DAPI anti-fade aqueous medium (Fluoroshield TM with DAPI, Thermo Fisher Scientific).

    Techniques: Expressing

    ZO-1 immunolabelling in the ileal mucosa. ( a – e ) Representative images of ZO-1 expression (green). Nuclei are counterstained with DAPI (blue). ( f ) Densitometric analysis of ZO-1-positive area. Exposure to hyperoxia significantly decreased ZO-1 expression compared to the normoxic controls ( a , c , f ). The treatment with BRL37344 at 3 mg/kg, but not at 1 mg/kg, had a protective effect ( c – f ). No significant changes were observed in the normoxia-exposed pups, treated or not with BRL37344 ( a , b , f ). Values are expressed as mean ± S.D. *** p < 0.001 hyperoxia versus normoxia; °°° p < 0.001 hyperoxia + BRL37344 versus normoxia; §§§ p < 0.001 hyperoxia + BRL37344 versus hyperoxia. Scale bar = 50 µm.

    Journal: Antioxidants

    Article Title: Protective Effects of Beta-3 Adrenoceptor Agonism on Mucosal Integrity in Hyperoxia-Induced Ileal Alterations

    doi: 10.3390/antiox13070863

    Figure Lengend Snippet: ZO-1 immunolabelling in the ileal mucosa. ( a – e ) Representative images of ZO-1 expression (green). Nuclei are counterstained with DAPI (blue). ( f ) Densitometric analysis of ZO-1-positive area. Exposure to hyperoxia significantly decreased ZO-1 expression compared to the normoxic controls ( a , c , f ). The treatment with BRL37344 at 3 mg/kg, but not at 1 mg/kg, had a protective effect ( c – f ). No significant changes were observed in the normoxia-exposed pups, treated or not with BRL37344 ( a , b , f ). Values are expressed as mean ± S.D. *** p < 0.001 hyperoxia versus normoxia; °°° p < 0.001 hyperoxia + BRL37344 versus normoxia; §§§ p < 0.001 hyperoxia + BRL37344 versus hyperoxia. Scale bar = 50 µm.

    Article Snippet: After the final washing step with PBS, the strips were transferred into a droplet of the anti-fade DAPI mounting medium (Fluoroshield TM with DAPI, Thermo Fisher Scientific, Waltham, MA, USA) dripped onto the microscope slide and oriented on their side to visualize the villus axis [ ].

    Techniques: Expressing

    E-cadherin immunolabelling in the ileal mucosa. ( a – e ) Representative images of E-cadherin expression (green; nuclei are counterstained blue with DAPI). ( f ) Densitometric analysis of E-cadherin-positive area. Exposure to hyperoxia significantly decreased E-cadherin expression compared to the normoxic controls ( a , c , f ). The administration of BRL37344 had a partially protective effect ( c – f ). No changes can be observed in the normoxic pups treated or not with BRL37344 ( a , b , f ). Values are expressed as mean ± S.D. *** p < 0.001 hyperoxia versus normoxia; °° p < 0.01 and °°° p < 0.001 hyperoxia + BRL37344 versus normoxia; §§ p < 0.01 hyperoxia + BRL37344 versus hyperoxia. Scale bar = 25 µm.

    Journal: Antioxidants

    Article Title: Protective Effects of Beta-3 Adrenoceptor Agonism on Mucosal Integrity in Hyperoxia-Induced Ileal Alterations

    doi: 10.3390/antiox13070863

    Figure Lengend Snippet: E-cadherin immunolabelling in the ileal mucosa. ( a – e ) Representative images of E-cadherin expression (green; nuclei are counterstained blue with DAPI). ( f ) Densitometric analysis of E-cadherin-positive area. Exposure to hyperoxia significantly decreased E-cadherin expression compared to the normoxic controls ( a , c , f ). The administration of BRL37344 had a partially protective effect ( c – f ). No changes can be observed in the normoxic pups treated or not with BRL37344 ( a , b , f ). Values are expressed as mean ± S.D. *** p < 0.001 hyperoxia versus normoxia; °° p < 0.01 and °°° p < 0.001 hyperoxia + BRL37344 versus normoxia; §§ p < 0.01 hyperoxia + BRL37344 versus hyperoxia. Scale bar = 25 µm.

    Article Snippet: After the final washing step with PBS, the strips were transferred into a droplet of the anti-fade DAPI mounting medium (Fluoroshield TM with DAPI, Thermo Fisher Scientific, Waltham, MA, USA) dripped onto the microscope slide and oriented on their side to visualize the villus axis [ ].

    Techniques: Expressing